Uji Kadar Total Tanin dari Ekstrak Daun Sembung (Blumea balsamifera) serta Toksisitasnya Dengan mentode BSLT
DOI:
https://doi.org/10.59188/jurnalsosains.v5i6.32260Keywords:
Blumea balsamifera, Tannins, ToxicityAbstract
Blumea balsamifera is one of the Asteraceae family known as Sembung leaves. B. balsamifera is often used in traditional medicine. This study aims to evaluate the potential biological activity of Sembung leaves (Blumea balsamifera) through the determination of total tannin levels and toxicity tests using the Brine Shrimp Lethality Test (BSLT) method. Leaf extraction is carried out by the maceration method using methanol solvent. The total tannin content was determined using the colorimetric method with the Folin-Ciocalteu reagent, resulting in a tannin content of 358.04 mg TAE/g of extract. The toxicity test showed an LC50 value of 56.58 μg/mL, which indicated that the methanol extract of B. balsamifera leaves was toxic. These results suggest that the high tannin content correlates with the toxicity properties of the extract, which has the potential to be further developed as a therapeutic agent.
References
Abunoya, J. I., Usman, U., Magister, S., Kimia, P., Mulawarman, U., Timur, K., Studi, P., Kimia, P., Mulawarman, U., & Timur, K. (2024). Uji fitokimia dan toksisitas ekstrak metanol jaringan kulit batang tanaman bintaro ( cerbera manghas l ) terhadap bibit ikan nila ( oreochromis niloticus ) phytochemical and toxicity testing of the methanol extract of the bark tissue of the bintaro plant . 2020, 101–106.
adar BakhshBaloch, Q. (2017). uji aktivitas antibakteri ekstrak kasar daun nipah (nypa fruticans) terhadap bakteri Aeromonas hydrophila DAN Streptococcus agalactiae. 11(1), 92–105.
Adolph, R. (2016). Penetapan kadar tanin dari bunga telang (clitoria ternatea l.) Secara permanganometri. 1–23.
Agustini, N. wayan S., & Setyaningrum, M. (2017). Identifikasi Senyawa Aktif dan Toksisitas Hayati Ekstrak n-heksana, Etil Asetat dan etanol Mikroalga Tetraselmis chuii Secara Brine Shrimp Lethality Test (BSLT). Warta Industri Hasil Pertanian, 34(1), 8. https://doi.org/10.32765/wartaihp.v34i1.4063
Ajrina, A. (2013). Uji Toksisitas Akut Ekstrak Metanol Daun Garcinia benthami Pierre Terhadap Larva Artemia salina Leach Dengan Metode Brine Shrimp Lethality Test ( BSLT ). Universitas Islam Negeri Syarif Hidayatullah Jakarta, 1–63.
Ananda, Z., Meilina, R., Sari, F., Husna, A., & Watani, N. (2022). Uji toksisitas subkronik ekstrak etanol daun putri malu ( mimosa pudica l .) Pada mencit galur wistar subcronic toxicity test of ethanol extract of leaves of putri malu ( Mimosa Pudica L .) IN WISTAR STREAM MICULES. 8(2).
Asran, A. N. (2021). Uji Toksisitas Subakut Daun Beruwas Laut (Scaevola tacadda L.) Terhadap Perubahan Hispatologi Lambung Tikus Putih Jantan. Skripsi, Universitas Hasanuddin.
Ernawati, K. L. (2018). KOMBUCHA TEA MENURUNKAN JUMLAH BAKTERI Streptococcus. Fakultas Kedokteran Gigi Universitas Mahasaraswati Denpasar, 6–10.
Filianty, F., Noor, E., Suryadarma, P., & Yuliasih, I. (2021). Eksplorasi Potensi Akar Millettia sericea sebagai Sumber Antioksidan Alami. Jurnal Teknotan, 15(2), 119. https://doi.org/10.24198/jt.vol15n2.9
Garnida, Y., & Hasnelly. (2018). “Pengaruh Perbandingan Sari Buah Mengkudu (Morinda citrifolia L) dan Filtrat Daun Rambutan (Nephelium lappaceum L) Terhadap Karakteristik Minuman Fungsional.” Pasundan Food Technology Journal, 5(3), 196–204.
Handayani, V., Najib, A., Syarif, R. A., Mahmud, A., Hamidu, L., & Ahmad, A. R. (2019). UJI TOKSISITAS EKSTRAK ETANOL TERPURIFIKASI BIJI MAHONI (Switenia mahagoni). Jurnal Fitofarmaka Indonesia, 6(2), 360–362. https://doi.org/10.33096/jffi.v6i2.511
Harman, M. P., & Ridwan, M. (2021). Phytochemical screening and LC₅₀ Brine Shrimp Lethality of Blumea balsamifera extract. Jurnal Farmasi Indonesia, 15(3), 45–52.
Hoque, M. B., Tanjila, M. J., Hosen, M. I., Hannan, M. A., Haque, P., Rahman, M. M., & Hasan, T. (2024). A comprehensive review of the health effects, origins, uses, and safety of tannins. Plant and Soil, May. https://doi.org/10.1007/s11104-024-06768-7
Idris, M. (2019). UJI TOKSISITAS AKUT EKSTRAK ETANOL DAUN SALAM (Syzygium polyanthum (Wight) Walp.) DENGAN METODE BSLT (Brine Shrimp Lethality Test). Medical Sains : Jurnal Ilmiah Kefarmasian, 4(1), 35–42. https://doi.org/10.37874/ms.v4i1.121
Karunia. (2016). Studi Tanaman Khas Sumatera Utara Yang Berkhasiat Obat. Jurnal Farmanesia, 4(June), 2016.
Kim, D.-I., Choe, S.-A., & Sin, K.-R. (2016). Interaction of tannin with bovine serum albumin by fluorescence spectrometry.
Listiana, L., Wahlanto, P., Ramadhani, S. S., & Ismail, R. (2022). Penetapan Kadar Tanin Dalam Daun Mangkokan (Nothopanax scutellarium Merr) Perasan Dan Rebusan Dengan Spektrofotometer UV-Vis. Pharmacy Genius, 1(1), 62–73. https://doi.org/10.56359/pharmgen.v1i01.152
Makiyah, A., & Tresnayanti, S. (2017). Uji Toksisitas Akut yang Diukur dengan Penentuan LD50 Ekstrak Etanol Umbi Iles-iles (Amorphophallus variabilis Bl.) pada Tikus Putih Strain Wistar. Majalah Kedokteran Bandung, 49(3), 145–155. https://doi.org/10.15395/mkb.v49n3.1130
Mustapa, M. A., Taupik, M., Mu, A., Suryadi, A., & Paneo, M. A. (2024). Penentuan Kadar Flavonoid Daun Sembung ( Blumea balsamifer ) menggunakan Metode Spektrofotometri UV-Vis. 4(2), 255–261. https://doi.org/10.37311/ijpe.v4i2.11284
Nessa, M., & Chen, Z. (2017). Tannin-rich methanolic extract of Blumea balsamifera: antioxidant properties. Journal of Ethnopharmacology, 202, 123–130.
Online, J., & Muhammadiyah, U. (2010). PENETAPAN KADAR TANIN DAUN RAMBUTAN (Nephelium lappaceum.L ) SECARA SPEKTROFOTOMETRI ULTRAVIOLET VISIBEL Dewi. 07(02), 1–11.
Pokhrel, S. (2024). Identifikasi bakteri endofit daun anting-anting (acalypha indica l.) Serta karakterisasi senyawa antibakteri secara in vitro & in silico dalam menghambat Klebsiella pneumoniae IDENTIFICATION. Αγαη, 15(1), 37–48.
Putri, D. A., & Santoso, H. (2019). Phytochemical analysis and BSLT toxicity of Blumea balsamifera leaf extracts. Jurnal Fitokimia Indonesia, 7(2), 77–84.
Putri, E. Z. (2019). Uji Efektivitas Ekstrak Daun Pandan Wangi (Pandanus amaryllifolius Roxb) Sebagai Insektisida Terhadap Lalat Rumah (Musca domestica). Universitas Islam Negeri Alauddin Makassar, 1–141.
Rafiqah, R., Mastura, M., & Hasibuan, M. . (2019). Uji Toksisitas Fraksi Etanol Tanaman Obat yang Digunakan Masyarakat Menggunakan Metode Brine Shrimp Lethality Test. Chemica : Jurnal Pendidikan Kimia dan Ilmu Kimia, 2(1), 14–20.
Rahmi, A., Afriani, T., & Aini. (2022a). Cytotoxic test of extract and fractions from Blumea balsamifera leaves using Brine Shrimp Lethality Test (BSLT). Jurnal Ilmiah Farmasi, 18(1), 10–18. https://doi.org/10.20885/jif.vol18.iss1.art3
Rahmi, A., Afriani, T., & Aini, A. (2022b). Cytotoxic test of extract and fractions from Blumea balsamifera leaves using Brine Shrimp Lethality Test (BSLT). Jurnal Ilmiah Farmasi, 18(1), 26–33. https://doi.org/10.20885/jif.vol18.iss1.art3
Rizka, D., Pulungan, A., Syahfitri, D., & Adelia, D. (2024). Daun Salam ( Syzygium polyanthum ) Rempah Khas Indonesia dengan Berbagai Manfaat Farmakologi : Literature Review. 4(3), 423–437. https://doi.org/10.37311/ijpe.v4i3.28452
Sari, N. M. (2023). Phytochemical and Antioxidant Activity of Blumea balsamifera Leaves: Implications for Tannin Content. Biology, Medicine & Natural Product Chemistry, 12(1), 273–280.
Subkhi Mahmasani. (2020). DAYA HAMBAT INFUSUM DAUN SEMBUNG (Blumea balsamifera) TERHADAP PERTUMBUHAN BAKTERI Escherichia coli DENGAN METODE DIFUSI CAKRAM. 274–282.
Sumiati, T., Effendi, F., & puspitasari, R. arifah. (2016). uji toksisitasekstrak daun sirsak (annona muricata l.) yang berpotensi sebagai antikanker. Jurnal Farmamedika (Pharmamedica Journal), 1(2), 85–91. https://doi.org/10.47219/ath.v1i2.22
Suryani, R. (2016). Phytochemical content and LC₅₀ evaluation of Blumea balsamifera leaf using Brine Shrimp Lethality Test. Indonesian Journal of Natural Products, 10(1), 1–8.
Tjondronegoro, M., & Arisanti, A. (2020). Total phenolics, tannins and cytotoxicity of Blumea balsamifera extracts. Asian Journal of Tropical Biomedicine, 13(4), 21–28.
Wannes, W. A., & Tounsi, M. S. (2021). Phytochemical composition and health properties of Lycium europaeum L.: A review. Acta Ecologica Sinica, 41(5), 390–401. https://doi.org/10.1016/J.CHNAES.2020.09.008
Widhiantara, I. G., Permatasari, A. A. A. P., Siswanto, F. M., & Dewi, N. P. E. S. (2018). Ekstrak Daun Sembung (Blumea balsamifera) Memperbaiki Histologi Testis Tikus Wistar yang Diinduksi Pakan Tinggi Lemak. Jurnal Bioteknologi & Biosains Indonesia, 5(2), 111–118. https://doi.org/10.29122/jbbi.v5i2.2868
Widyasari, R., Yuspitasari, D., Wildaniah, W., & Cahayuni, R. (2018). Uji Toksisitas Akut Ekstrak Metanol Kulit Buah Jeruk Sambal (Citrus microcarpa Bunge) Terhadap Larva Artemia salina L. Dengan Metode Brine Shrimp Lethality Test (BSLT). Medical Sains : Jurnal Ilmiah Kefarmasian, 3(1), 51–58. https://doi.org/10.37874/ms.v3i1.64
Wulandari, E., & Prasetyo, B. (2018). Phytochemical and BSLT toxicity of Indonesian medicinal plant Blumea balsamifera. Jurnal Biologi Indonesia, 14(2), 99–105.
Xu, J., Zhao, Y., & Liu, X. (2016). Blumea balsamifera extract: antioxidant and hepatoprotective effects. In Phytochemical & Pharmacological Review.
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